Cellular and Extracellular Proteome of the Animal Pathogen Corynebacterium silvaticum, a Close Relative of Zoonotic Corynebacterium ulcerans and Corynebacterium pseudotuberculosis.

Jens Möller,Jörg Hofmann,Svenja Schorlemmer,Andreas Burkovski

doi:10.3390/proteomes8030019

Jens Möller, Jörg Hofmann + Show 2 more

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https://doi.org/10.3390/proteomes8030019

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Journal: Proteomes	Publication Date: Aug 12, 2020
Citations: 9	License type: CC BY 4.0

Affiliation: University of Erlangen-Nuremberg

Abstract

Corynebacterium silvaticum is a newly described animal pathogen, closely related to the emerging human pathogen Corynebacterium ulcerans and Corynebacterium pseudotuberculosis, a major pathogen of small ruminants. In this study, proteins of a whole cell and a shaving fraction and the exoproteome of C. silvaticum strain W25 were analyzed as a first proteome study of this species. In total, 1305 proteins were identified out of 2013 proteins encoded by the W25 genome sequence and number of putative virulence factors were detected already under standard growth conditions including phospholipase D and sialidase. An up to now uncharacterized trypsin-like protease is by far the most secreted protein in this species, indicating a putative role in pathogenicity. Furthermore, the proteome analyses carried out in this study support the recently published taxonomical delineation of C. silvaticum from the closely related zoonotic Corynebacterium species.

Highlights

The genus Corynebacterium, first described in 1886 as a group of Gram-positive, club- or rod-shaped bacteria [1], comprises between 142 and 145 different species today [2,3].These were often isolated from human or animal material and are frequently of medical or veterinary importance [4,5]
Cases of diphtheria associated with C. ulcerans infections have outnumbered cases attributed to C. diphtheriae in European countries [14] and
Two other virulence factors Vsp1 and Vsp2 contain a similar trypsin-like serine protease domain as A0A5C5F2T7, which may indicate a prominent role in pathogenicity

Summary

Introduction

The genus Corynebacterium, first described in 1886 as a group of Gram-positive, club- (greek: korune) or rod-shaped bacteria [1], comprises between 142 and 145 different species today [2,3]. These were often isolated from human or animal material and are frequently of medical or veterinary importance [4,5]. Corynebacterium diphtheriae, the type strain of the genus, is the etiological agent of diphtheria [6,7,8] and most likely the best investigated pathogenic Corynebacterium species due to its life-threatening properties [9]. Cases of diphtheria associated with C. ulcerans infections have outnumbered cases attributed to C. diphtheriae in European countries [14] and

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