Abstract
The effect of temperature variation on the speed of amoeboid movement ofNaegleria gruberi, a common soil protozoon, across a planar glass substrate under different electrolyte conditions (deionized water and 10 mM NaCl) has been measured. Cellsubstratum interactions of this amoeba were already known to differ at 20°C in these two media. While motility was evident at 8°C in these two media, speed of locomotion increased with temperature in both media, doubling between 20°C and 30°C, only to fall off sharply above 35°C. The rate of change of cell-substratum contact at both 20°C and 30°C was studied by reflexion interference microscopy. In the light of this data possible models for amoeboid locomotion involving associated contact and focal contacts were developed particularly in relation to a possible steering role played by the anterior region ofNaegleria.
Published Version
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