Abstract
The ability of adenoviruses to replicate viral DNA and produce infectious virus particles in tissue culture cells blocked with n-butyrate has been examined. Cells are treated with millimolar amounts of n-butyrate, gradually stopping cell division and DNA synthesis; the block is reversible when n-butyrate is removed. When HeLa cells which have been thus inhibited are infected with human adenovirus type 5 (Ad5) in the continued presence of inhibitor, the virus adsorbed and penetrated the cells normally and viral DNA synthesis ensued. A normal rate and time course of DNA synthesis was observed, and butyrate-treated cells yielded the same amount of infectious virus as uninfected cells. Adenoviral DNA synthesis occurred without detectable stimulation of cellular DNA replication or of histone synthesis. The mechanism of butyrate inhibition of cellular DNA replication and unique features of adenovirus replication are discussed.
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