Abstract
The aim of this study was to investigate the effect of intramammary infusions of natural composition GLP 810 with immunomodulating properties on the local nonspecific cellular and humoral immune response in cows with subclinical mastitis. The composition GLP 810 consists of lactic acid, lysozyme, glycopeptides, and 0.9% solution of NaCl. The following parameters were studied: (1) leukocyte differential distribution in milk, (2) expression of cytokines in milk leukocytes, (3) antibacterial activity, and (4) milk quality. Nineteen mammary glands in five lactating cows were infused with 10 mL of GLP 810, and nineteen other glands from five control cows were treated with 10 mL 0.9% NaCl. The results showed that after intramammary administration of the composition GLP 810 three times with 48 h intervals, the following effects on leukocyte populations in milk were observed: (1) an increase in the number of polymorphonuclear leukocytes and lymphocytes and (2) a decrease in the number of macrophages. A reduction in the number of pathogenic bacteria was also detected. The analyses of tumour necrosis factor-alpha, interleukin-10, and beta-defensin-2 revealed that the production of the aforementioned cytokines significantly increased, whereas no significant effects on interleukin-1 and caspase-6 expression in milk leukocytes were recorded. However, there were significant differences between mammary glands with high and low milk somatic cell count. The results suggest that the composition GLP 810 has an immunomodulatory effect on mammary glands and it could be used for improving the immune response in cows with subclinical mastitis during lactation.
Highlights
Mastitis or udder inflammation is a disease of major economic importance in dairy industry worldwide as discussed elsewhere [1, 2]
The increase in polymorphonuclear leukocytes (PMNL) count continued until the 14th day and probably longer in the experimental group mammary glands, while the number of PMNL was decreased on the 14th day in the control group milk with no significant difference from the baseline
In order to evaluate the effect of the immunomodulatory composition GLP 810 on the functional state of the bovine nonspecific cellular immune system locally in the mammary glands, we evaluated the number of leukocyte populations
Summary
Mastitis or udder inflammation is a disease of major economic importance in dairy industry worldwide as discussed elsewhere [1, 2]. The animal immune status plays a key role in the development of mastitis after an invasion of bacteria into mammary glands; the maintenance of animal health by encouraging the natural immunological defence including the use of immunomodulators could serve as an important alternative to antibiotic therapy. The fight against the development of bacterial resistance is based on prudent and rational use of antibiotics, and it is a priority task in the human health sector and veterinary medicine worldwide, but alternative methods for the treatment of mastitis are needed to carry out this task. The aim of this study was to investigate the effect of intramammary infusions of natural composition GLP 810 with immunomodulating properties on the local nonspecific cellular and humoral immune response in cows with subclinical mastitis
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