Abstract
BackgroundImage-based screens can produce hundreds of measured features for each of hundreds of millions of individual cells in a single experiment.ResultsHere, we describe CellProfiler Analyst, open-source software for the interactive exploration and analysis of multidimensional data, particularly data from high-throughput, image-based experiments.ConclusionThe system enables interactive data exploration for image-based screens and automated scoring of complex phenotypes that require combinations of multiple measured features per cell.
Highlights
Image-based screens can produce hundreds of measured features for each of hundreds of millions of individual cells in a single experiment
The scientific community has only begun to scratch the surface of computationally extracting the rich information visible in fluorescence microscopy images of cell samples [1]
We recently developed open-source image analysis software, CellProfiler, which measures a rich set of cellular features in images, such as size, shape, and staining patterns including intensity, texture, and colocalization [4,5]http://www.cellprofiler.org
Summary
Image-based screens can produce hundreds of measured features for each of hundreds of millions of individual cells in a single experiment. The scientific community has only begun to scratch the surface of computationally extracting the rich information visible in fluorescence microscopy images of cell samples [1]. We recently developed open-source image analysis software, CellProfiler, which measures a rich set of cellular features in images, such as size, shape, and staining patterns including intensity, texture, and colocalization [4,5]http://www.cellprofiler.org. This tool has been useful for extracting image-based measurements to score sophisticated screens [6,7,8], with many more in progress. For analysis of small or very simple experiments, spreadsheet programs like Microsoft Excel are sufficient, and useful open-source tools exist for analysis and exploration of data from high throughput screens in general [9,10,11,12]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
