Abstract
From the biotechnological viewpoint, the enzymatic disintegration of plant lignocellulosic biomass is a promising goal since it would deliver fermentable sugars for the chemical sector. Cellobiose dehydrogenase (CDH) is a vital component of the extracellular lignocellulose-degrading enzyme system of fungi and has a great potential to improve catalyst efficiency for biomass processing. In the present study, a CDH from a newly isolated strain of the agaricomycete Coprinellus aureogranulatus (CauCDH) was successfully purified with a specific activity of 28.9 U mg−1. This pure enzyme (MW = 109 kDa, pI = 5.4) displayed the high oxidative activity towards β-1–4-linked oligosaccharides. Not least, CauCDH was used for the enzymatic degradation of rice straw without chemical pretreatment. As main metabolites, glucose (up to 165.18 ± 3.19 mg g−1), xylose (64.21 ± 1.22 mg g−1), and gluconic acid (5.17 ± 0.13 mg g−1) could be identified during the synergistic conversion of this raw material with the fungal hydrolases (e.g., esterase, cellulase, and xylanase) and further optimization by using an RSM statistical approach.
Highlights
As alternative feedstock for biotechnological and industrial applications, lignocellulosic materials like wood and agricultural residues become more and more economically important, not least against the background of intensified utilization of biomass in the sense of the biorefinery concept and the idea of sustainable development
The results revealed 100% identity with the sequences of species Coprinellus aureogranulatus GQ249274
Based on this genetic identification, and additional microscopic characteristics, the strain MPG14 was classified as Coprinellus aureogranulatus (Psathyrellaceae, Agaricales, Agaricomycetes, Basidiomycota; Fig. 1 and Additional file 1: Fig. S1), which is a new species for Vietnam
Summary
As alternative feedstock for biotechnological and industrial applications, lignocellulosic materials like wood and agricultural residues become more and more economically important, not least against the background of intensified utilization of biomass in the sense of the biorefinery concept and the idea of sustainable development. It is assumed to play a role in reinforcement of manganese peroxidase, lignin peroxidase, laccase and cellulase, reducing aromatic radicals formed during lignin degradation, producing lignocellulose-modifying hydroxyl radicals and catalyzing quinone reduction as detoxification mechanism during fungal plant attack. Due to their bioelectrochemical properties, CDHs have a broad application potential in bioprocesses such as electrobiocatalysis, bioremediation, and in enzyme-based biofuel cells [4, 5, 7, 8]. Numerous CDHs have been isolated and characterized from fungal sources including white-rot fungi, such as Phanerochaete chrysosporium [9], Trametes hirsuta [10], Schizophyllum commune [11], Sporotrichum pulverulentum [12], soft-rot fungi like Chaetomium cellulolyticum [13], Sporotrichum thermophile [14], Humicola insolens [15], and the brown-rot fungus Coniophora puteana [16]
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