Abstract
Physically induced stem cells differentiation is gaining tremendous attention recently. Studies showed that matrix elasticity can induce human mesenchymal stem cells (hMSCs) differentiation [1]. In this work, we focused on how the cell-matrix interaction will affect hMSCs differentiation. First, the effect of matrix elasticity on hMSCs was explored, and it was found that besides the matrix compliance, selection of coating protein and concentration used play an important role in triggering the matrix elasticity effect on hMSCs differentiation. Collagen type I and human fibronectin could improve matrix compliance induced differentiation while gelatin did not have such effect. Secondly, we found that when the focal adhesion size and cytoskeleton development were controlled through micropatterning, the hMSCs differentiation was also affected.
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