Cell-Cycle Phase Analysis in Glucose-Limited Continuous Cultivation for the Effective Production of Antibody

Abstract

According to the obseravation of DNA duplication in a eukaryotic cell, the cell-cycle is divided into four discrete phases, G1 (gap period I), S (DNA synthesis), G2 (gap period II), and M (Mitotic). In a eucaryotic cell, most of the protein synthesis is dependent on the cell-cycle phase. The antibody production is also dependent on the cell-cycle from the results of synchronous culture experiments on lymphocyte [1] and myeloma cells [2]. Therefore, in a hybridoma cell, the antibody production is also considered to be cell-cycle dependent. The cell-cycle phase analysis has been enabled by the development of flow cytometry without using syncronous culture nor a radio isotope. In this report, we investigated the relationship between cell-cycle phase and antibody production by flow cytometry using synchronous cutlture or glucose-limited continuous culture.