Abstract
The cell cycle and intraclonal variation were studied in the bipolar centric diatom Biddulphiopsis titiana (Grunow) v. Stosch & Simonsen. Three differently sized subclones, small [S], large [L], and extra large [XL], and a tripolar strain were all derived from a single cell and cultivated under identical conditions. No difference in ultrastructure was apparent, except for the additional pole in the tripolar cells, coupled to a reduction in the number of rimoportulae per pole. Cell size parameters were compared using SDS-cleaned cell walls of 5 different cell-cycle stages. In the living cell, except during cell division, plastids perform karyostrophic movement (i.e. to the nucleus) during the dark phase or whenever exposed to strong incident light. Plasmolysis can clearly be distinguished from a contraction of the protoplast in response to physiological or mechanical shock. The reactions differ in degree and speed. Preprophase is correlated with cell elongation and the formation of the second girdle band o...
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