Abstract
The LEAP™ (Laser-Enabled Analysis and Processing) platform combines in situ imaging with laser manipulation to identify, select and monitor expansion of high recombinant protein secreting clones. Transfected populations and single-cell clones were generated using the Glutamine Synthetase (GS) System licensed from Lonza Biologics. CHOK1SV single-cell clones that stably express human anti-rabies SO57 IgG were expanded and characterized for growth and productivity. These clones were also analyzed using the Cell Xpress™ software module on the LEAP™ platform. Secretion average intensity values in Cell Xpress™ analysis exhibited good correlation (R 2 = 0.84) with peak IgG volumetric productivities in shake-flask growth and expression experiments. In a parallel study, secretion population dynamics during single-cell clone expansion was studied using Cell Xpress™. Day 7 after cloning, the clones exhibited different degrees of secretion heterogeneity. In conclusion, Cell Xpress™ enables high-throughput multi-parameter clone analysis and selection based on IgG secretion intensity and heterogeneity. Single-cell clones may develop secretion heterogeneity during expansion, which may contribute the post-expansion productivity and/or clone stability.
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