Cell Weight, Chlorophyll a Content and Total Biomass Production of Spirulina platensis: Impact of Culture Media and Affiliated Correlation

Abstract

This experiment was inquired to apprehend the associated relationship among the cell weight, chlorophyll a and total biomass production of Spirulina platensis grown in different concentration of digested rotten guava medium (DRGM) and kosaric medium (KM). This study was carried out to disclose the culture potentiality of different substrates and their impact on production through the assessment of their consequential correlation. The completely randomized design (CRD) was taken into consideration to accomplish the experiment in laboratory condition. The maximum cell weight was assumed in KM (12.41 ± 0.23) followed by 0.816 ± 0.0017, 0.813 ± 0.0013 and 0.807± 0.0011 mg/L in supernatant of 60%, 20% and 40% DRGM, respectively on the 10th day of culture. The correlation (P< 0.01) with the chlorophyll a content (r = 0.745) and total biomass (r = 0.741) revealed significant association and strong correlation with the obtained cell weight in these culture media. In kosaric medium, the growth performance was significantly higher (P<0.01) than that of grown in the different concentration of DRGM (60%, 40% and 20%). The content of chlorophyll a grown in KM (10.54 ± 0.33 mg/L) was significantly higher (P< 0.01) in comparison of 60% (0.863 ± 0.0018), 40% (0.769 ± 0.0015) and 20% DRGM (0.771 ± 0.13). The highest biomass of S. platensis was acquired in KM (705.52 ± 2.59 mg/L) and varied significantly (P < 0.05) than that of cultured in supernatants of 20% (67.76 ± 0.42), 40% (51.45 ± 0.26) and 60% (57.74 ± 0.26) DRGM. The culture techniques in DRG medium may dwindle the cost of production in comparison with KM and might be considered as a media for Spirulina platensis cultivation.