Cell wall‐degrading enzymes and pectin solubility and depolymerization in immature and ripe watermelon (Citrullus lanatus) fruit in response to exogenous ethylene

Abstract

Watermelon fruit have been shown to be extremely sensitive to exogenous ethylene, exhibiting acute symptoms of whole‐fruit softening and placental‐tissue water‐soaking following short periods of exposure to the gas. This study addressed the firmness, specific activities of cell wall hydrolases, and solubility and molecular mass properties of polyuronides in placental tissue in response to treatment of intact fruit with ethylene. Watermelon fruit were harvested at the immature and full‐ripe stages and exposed to 50 µl l−1 ethylene for 6 days at 20°C. The firmness of placental tissue from ethylene‐treated ripe and immature fruit decreased nearly 80% during 6 days of ethylene exposure, whereas the firmness of placental tissue from fruit maintained in air remained relatively constant up to day 3 and then decreased slightly (12%) during the following 3 days of storage. Although ethylene treatment in some cases influenced the levels of extractable placental‐tissue polygalacturonase (EC 3.2.1.15), pectinmethylesterase (EC 3.2.1.11), and α‐(EC 3.2.1.22) and β‐galactosidase (EC 3.2.1.23) specific activities, these effects were not observed for fruit of both developmental stages and appeared not to be directly involved in the water–soaking syndrome. Symptoms of water‐soaking were accompanied by increases in the levels of water‐ and CDTA (trans‐1,2‐cyclohexanediamine‐N,N,N',N′‐tetraacetic acid)‐soluble polyuronides and significant molecular mass downshifts in polyuronides in both immature and ripe watermelon fruit. Polyuronide depolymerization in ethylene‐treated ripe fruit was extensive. The parallel trends of enzyme changes in ethylene‐ compared with air‐treated fruit indicate that extractable enzyme levels are not associated with development of the water‐soaking disorder. The potential involvement of membrane dysfunction in the water‐soaking phenomenon is discussed.