Cell wall proteins of flax phloem fibers

Abstract

The cell wall of the same type - phloem fibers (Linum usitatissimum L.), active forming the thick secondary cell wall, - was obtained. Weakly bound cell wall proteins of phloem fibers were extracted and it subsequent separation and obtaining mass spectra was carried out. For identification and attachment of identified proteins to a specific cell compartments a variety ofbioinformatics methods was used. Were identified 93 proteins, many of which were defined as predicted, putative or hypothetical. At the same 21 proteins were identified as cell-wall protein. The absence of such marker proteins of the primary cell wall as xyloglucan-endotransglycosilase, expansins indirectly confirms that in the sample for extraction of proteins dominated the secondary cell wall.