Abstract
Cultures of bean (Phaseolus vulgaris L.) callus were adapted for growth in a 12 μM concentration of the herbicide isoxaben; 1 200‐fold greater than the I50 calculated. Tolerant calluses grew slower, were browner and harder than nontolerant ones and formed large globular corpuscles on their surface. Cell wall fractionation of tolerant cells indicated that the mechanism of adaptation depends on the ability of these cells to divide and expand with a modified wall. The walls of tolerant cells growing on isoxaben differ from those of nontolerant cells by having reduced levels of cellulose and hemicelluloses and a higher uronic acid content. These differences are already found in adaptations to low concentrations of isoxaben and keep to the highest adaptation levels tested without any great change.
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