Abstract
It has been discussed that the increase in the intake ratio of calcium and magnesium (Ca/Mg ratio) increases the risk of the ischemic heart disease. Though there are some reports on Ca/Mg ratio concerning the ischemic heart disease in animal experiments, there are few reports of experiments on cultured cells. Therefore, we investigated the influence of various ratios of Ca/Mg on the cell viability of cultured fibroblasts at various passage numbers. As a result, cell viability of cultured fibroblasts at passage number 14 or more significantly decreased with rises in Ca/Mg ratios. Furthermore, it was shown that this phenomenon was related to Ca/Mg ratios and not to rising Ca concentrations used in this report. Additionally, it was shown that the supplementation of DSW collected from Izu-Akazawa, Japan which did not change the Ca/Mg ratio, to cells in a state of decreased cell viability due to the increased Ca/Mg ratios showed an inhibition against decreasing their cell viability. Considering these results, it is suggested that the application of DSW will be a promising approach on health maintenance because the Ca/Mg ratio has been an increasing trend along with the diversification of the Japanese diet.
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