Cell type specific expression and regulation of murine interferon α and β genes

Abstract

The differential and cell type specific expression of various murine IFN α genes and IFN β was examined by S1 nuclease protection assays in M-CSF cultured C57BL/6 mouse bone marrow macrophages and L929 fibroblasts. In Newcastle disease virus (NDV) induced macrophages, IFN β, α 2, α 4, and α 1 mRNAs were the predominant species, whereas IFN α 6 and α 9 transcripts accounted for only 5% of total IFN α mRNAs. In L929 cells, only IFN β α 2, and α 4 genes were expressed efficiently following NDV induction and IFN α 9 mRNA was always below detectable level. Induction of macrophages with the synthetic inducer 10-carboxymethyl-9-acridanone resulted in small amounts of IFN α 2, α 4, and α 6 mRNAs and the IFN β mRNA level was about 100-fold higher. Macrophages and L929 cells especially differed in the kinetics of IFN gene induction in that macrophages showed a much earlier transient expression of all IFN mRNA species. Additionally, IFN transcripts were degraded much faster in macrophage cultures than in L929 cells. The IFN response of macrophages is thus characterized by a highly efficient control, providing a rapid onset and a rapid decline of IFN production, which limits release of IFN to a short time interval.