Abstract
To evaluate the importance of stem cells derived from adipose tissue in reducing graft inflammation in a murine model of allogeneic heterotopic tracheal transplant. We performed a heterotopic tracheal allografting in dorsal subcutaneous pouch and systemically injected 5x105 mesenchymal stem cells derived from adipose tissue. The animals were divided into two groups according to the time of sacrifice: T7 and T21. We also carried out histological analysis and digital morphometry. The T7 animals treated with cell therapy had median obstructed graft area of 0 versus 0.54 of controls (p = 0.635). The treated T21 subjects had median obstructed graft area of 0.25 versus 0 in controls (p = 0.041). The systemically injected cell therapy in experimental murine model of bronchiolitis obliterans did not reduce the severity of the allograft inflammation in a statistically significant way in seven days; Conversely, in 21 days, it increased the allograft inflammatory process.
Highlights
Lung transplantation has become a viable alternative for many terminal pulmonary diseases
Considering that Bronchiolitis obliterans (BO) is a disease of inflammatory nature, which limits the survival of lung transplantation and therapeutic options are limited, we prepared an experimental study to assess the importance of stem cells derived from adipose tissue in reducing the graft inflammatory process in a murine model of allogeneic heterotopic tracheal transplant
To obtain mesenchymal stem cells derived from adipose tissue, the C57BL6 mice were killed by cervical dislocation on the Animal Experimentation Unit (UEA)
Summary
Lung transplantation has become a viable alternative for many terminal pulmonary diseases. MSC have been tested in clinical trials in humans graft versus host disease scenarios after transplantation of allogeneic hematopoietic stem cells and inflammatory bowel disease[8,9]. Considering that BO is a disease of inflammatory nature, which limits the survival of lung transplantation and therapeutic options are limited, we prepared an experimental study to assess the importance of stem cells derived from adipose tissue in reducing the graft inflammatory process in a murine model of allogeneic heterotopic tracheal transplant.
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