Cell survival determined by colony formation assay after acute or chronic exposure to MMC

Abstract

Copyright information: Taken from Disparate requirements for the Walker A and B ATPase motifs of human RAD51D in homologous recombinationNucleic Acids Research 2006;34(9):2833-2843.Published online 22 May 2006PMCID:PMC1464408.© The Author 2006. Published by Oxford University Press. All rights reserved () Parental lines and high-expressing transfectants carrying Walker A-motif mutations were exposed to MMC for 60 min. AA8, solid squares; 51D1Lox (+), open squares; 51D1 () expressing wild-type human RAD51D (WT2), half-filled squares; 51D1 expressing Walker A-motif mutant, clone GAT4, solid diamonds; 51D1 expressing Walker A-motif mutant, clone GRT6, solid circles. () Parental, mutant (clone: 51D1) and low-expressing transfectants carrying Walker A-motif mutations were exposed to MMC for 60 min. Mutant 51D1 (), open diamonds; 51D1 expressing Walker A-motif mutant, clone GAT2, solid diamonds; 51D1 expressing Walker A-motif mutant, clone GRT2, solid triangle; 51D1 expressing Walker A-motif mutant, clone GRT3, solid circle; for AA8, 51D1Lox and 51D1 expressing wild-type RAD51D (here clone WT1) symbols same as in (A). Horizontal line indicates D survival. () Survival of colony-forming ability in response to continuous MMC exposure of cells and transfectants expressing either wild-type RAD51D (WT; open squares), K113R (open triangles, K113A (open circles), V203E (open diamonds), D206A (closed triangles). AA8 cells (closed circles) and cells transfected with the empty vector (pcDNA3.1/; black cross) are shown for comparison. Error bars that are visible are ± 1 standard deviation from three independent experiments.