Cell surfaces in plant micro-organism interactions. VIII. Increased proteinase inhibitor activity in melon plants in response to infection by Colletotrichum lagenarium or to treatment with an elicitor fraction from this fungus

Abstract

Proteinase inhibitor activity had increased sharply in melon seedlings infected by Colletotrichum lagenarium 3 days after inoculation. The activity was associated with heat stable proteins and was effective against the protease produced by the fungus as well as against trypsin. Treatment of healthy melon leaves with an elicitor of ethylene isolated from the pathogen, resulted in a three-fold increase in proteinase inhibitor activity after 24 h. Ethylene production increased early in elicitor-treated leaves and may be involved in the elicitation of proteinase inhibitor activity. In the presence of aminoethoxyvinylglycine, an inhibitor of ethylene biosynthesis, both elicitor-induced ethylene and, to a lesser extent, elicitor-induced proteinase inhibitor activity were inhibited. In contrast, 1-aminocyclopropane-1-carboxylic acid, the direct precursor of ethylene, triggers proteinase inhibitor activity. It is concluded that ethylene is involved in the elicitation of proteinase inhibitor activity, but its exact role remains to be defined.