Cell Surface Determinants Associated with the Interaction of Human CTL Clones and Autologous Tumor Cells

Abstract

Activation of peripheral blood lymphocytes (PBL) of tumor patients by mitogenic lectins (1), allogeneic lymphocytes (2) or autologous tumor cells (3–5) has been shown to result in the generation of cytotoxic lymphocytes that are able to lyse autologous tumor cells in vitro. These findings indicate that in these lymphocyte bulk cultures clones of cytotoxic lymphocytes with auto-tumor reactivity are generated. The aim of the studies described here was 1) to isolate cytotoxic T lymphocyte (CTL) clones with auto-tumor reactivity from mixed lymphocyte tumor cell cultures (MLTC) in which PBL of melanoma of B-lymphoma patients were cocultivated with irradiated autologous melanoma or autologous B-lymphoma cells (6,7); 2) to analyse the specificity of these CTL clones, and 3) to study the specific recognition- and interaction structures associated with CTL clone — autologous tumor cell interaction.KeywordsMelanoma CellAutologous Tumor CellCytotoxic ReactivityAutologous Melanoma CellCell Surface DeterminantThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.