Cell-specific polymorphism of acid phosphatase in human blood cells: their functional and leukemic variants.

Abstract

AbstractThe observation that the cytochemical patterns of certain enzymes represent a stable marker for the identification of blood cells and resist functional stimuli as well as malignant transformation prompted the study of cell-specific polymorphism of acid phosphatase (EC 3.1.3.2) in human blood cells. Normal and leukemic human T and B lymphocytes as well as normal and leukemic blood monocytes and human alveolar macrophages as a functional derivative of blood monocytes were separated and subjected to purity control. Lysosomes. collected after cell cavitation, were solubilized and the particle-free supernatant was subjected to a direct assay of acid phosphatase using p-nitrophenylphosphate. Aliquot samples were analyzed by thin-layer isoelectric focusing and the banding of the acid phosphatase was recorded on gels using naphthol AS-BI phosphate after having demonstrated that on a quantitative term a positive correlation exists between the results with both substrates. The banding patterns proved to be ...