Cell Specific Early Dysfunctional Gene Clusters in the APPNL-G-F (APPKI) Alzheimer's Model

Abstract

Alzheimer's Disease (AD) occurrence is expected to triple by the year 2060. Due to a lack of the understanding of causative mechanisms we are devoid of effective therapies. We have explored the hippocampus and cortex of the 2-month old APPNL-G-F (APPKI) using 10× Genomics Chromium Single Cell Multiome Assay for Transposase Accessible Chromatin (ATAC) + Gene Expression (GEX) to define at the transcriptome level, cell specific alterations in immune, lipid , metabolic and neurodevelopment related gene clusters. Additionally, we have assessed DNA-methylation to uncover related CpG islands and gene promoters.