Abstract
To investigate the effects of guanine nucleotide-binding regulatory proteins (G proteins) on hormonal regulation of prolactin (PRL) synthesis and secretion, the qualitative distribution of G protein α-subunits and their mRNAs was studied in three functionally different pituitary tumour cell lines (GH cells) and normal rat pituitary tissue. Levels of basal and modulated adenylyl cyclase (AC) and phospholipase C (PLC) activities are also included. GH cells and pituitary tissue contained various amounts of mRNAs and protein for G sα, G i−2α, G i−3α and G 0α, while mRNA for G i−1α was only detected in normal pituitary tissue. G zα/G xα mRNA was expressed in all pituitary cell lines as well as in pituitary tissue. G 0α mRNA and G zα/G xα mRNA displayed size heterogeneity. These findings may have importance in the understanding of hormone regulation of second messenger systems.
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