Abstract
Argon plasma treatment was used to modify the surface of atelocollagen films using a plasmochemical reactor. To evaluate the effects of the treatment, the untreated and treated samples were characterized by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR), Scanning Electron Microscopy (SEM) imaging, and X-ray Photoelectron Spectroscopy (XPS) techniques. Cell growth was carried out by culturing human immortalized keratinocyte (HaCaT) cells and proliferation was measured via MTT assay. It was observed that argon plasma treatment significantly enhanced the extent of cell proliferation, which was ascribed to the favourable role of plasma treatment in inducing surface oxygen-containing entities together with increasing surface roughness. This can be considered as a potentially promising approach for tissue regeneration purposes.
Highlights
Collagen is a fibrillar protein that exists in most mammalian tissues
X-ray photoelectron Spectroscopy (XPS) spectra have been recorded in order to gain an insight into the chemical modifications of the treated surface
The data shows a considerable increase in the oxygen content subsequent to the argon plasma treatment which is reflected as a raise in O/C ratio
Summary
Collagen is a fibrillar protein that exists in most mammalian tissues. It constitutes ca. 25% of whole-body protein content. Skin consists of different types of cells such as keratinocytes, melanocytes, and fibroblasts [2] It is well established through wound healing, transplantation, and cell culture studies that human immortalized keratinocyte (HaCaT) cells play a crucial role in epidermal tissue regeneration, since they are spontaneously transformed to human keratinocytes which have the traits of basal epidermal keratinocytes and can be delivered in deep burns. A systematic study of argon plasma treatment effects on HaCaT keratinocyte cell response of atelocollagen films with a view to designing a novel material potentially suitable for tissue engineering applications is undertaken. This is accomplished via surface probe techniques together with pertinent biological assays
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