Cell proliferation markers at the invasive tumor front of oral squamous cell carcinoma: comparative analysis in relation to clinicopathological parameters of patients.

Aurita Veronica Beovide Cortegoso,Fernanda Visioli,Pantelis Varvaki Rados,Alessandra Sellinger Magnusson,Chris Krebs Danilevicz,Natalia Koerich Laureano,Alessandra Dutra Da Silva

doi:10.1590/1678-7757-2016-0238

Abstract

Objectives To evaluate the number of AgNORs per nucleus and the expression of Ki-67 at the tumor invasion front (TIF) in relation to clinical parameters (TNM), TIF classification and the prognosis of oral squamous cell carcinomas in an Uruguayan population.Material and Methods This study was conducted through a retrospective survey from 2000 to 2010 at the National Institute of Cancer Montevideo, Uruguay and included 40 patients. The samples were obtained from the resection of the tumor and the TIF was defined according with Bryne, et al.5 (1992). Expression of Ki-67 was assessed by the percentage of positive tumor cells and the AgNOR was recorded as the mean AgNOR (mAgNOR) and the percentage of AgNOR per nucleus (pAgNOR). All analyzes were performed by a blinded and calibrated observer.Results No statistically significant association was observed between immunostaining of Ki-67 and AgNOR with the different types of TIF, regional metastasis and patients prognosis, however it was observed an increase in Ki-67 expression associated with worse patient’s clinical staging, although not statistically significant.Conclusions Our results suggest that proliferation markers as AgNOR and Ki-67 are not prognostic markers at the tumor invasive front of carcinoma of oral squamous cell.

Highlights

Oral cancer is a important global health disease with more than 300,000 new cases annually, totalizing over 275,000 cases and 128,000 deaths per year and the morbidity and mortality rates have not improved in the past decades18
Studies have shown different patterns of oral squamous cells carcinomas in the various regions of the world, but few studies have evaluated this neoplasm in the Uruguayan population2,9
It is believed that the most useful site for predicting prognosis is the forehead area of tumor invasion front (TIF) as it would presumably reside PRUH DJJUHVVLYH FHOOV 0RUSKRORJLFDOO\ 7,) UHÀHFWV various molecular interactions that are crucial for the progression of cancer: increased angiogenesis, alteration of adhesion molecules, overproduction of enzymes that degrade the extracellular matrix and an increase in the expression of proteins related to cell proliferation1,4,5

Summary

Introduction

Oral cancer is a important global health disease with more than 300,000 new cases annually, totalizing over 275,000 cases and 128,000 deaths per year and the morbidity and mortality rates have not improved in the past decades. Studies have shown different patterns of oral squamous cells carcinomas in the various regions of the world, but few studies have evaluated this neoplasm in the Uruguayan population. In Uruguay oral squamous cell carcinoma (OSCC) is diagnosed late, which provides poor prognosis and low survival rate for this disease. The histological features of the OSCC can be widely different from one area to another within the same tumor. It is believed that the most useful site for predicting prognosis is the forehead area of tumor invasion front (TIF) as it would presumably reside PRUH DJJUHVVLYH FHOOV 0RUSKRORJLFDOO\ 7,) UHÀHFWV various molecular interactions that are crucial for the progression of cancer: increased angiogenesis, alteration of adhesion molecules, overproduction of enzymes that degrade the extracellular matrix and an increase in the expression of proteins related to cell proliferation. The analysis of morphological features such as: degree of keratinization, nuclear polymorphism, pattern of invasion and lymphocytic LQ¿OWUDWLRQRIWKH7,)GHPRQVWUDWHGSURJQRVWLFYDOXH as a supplement to the TNM4,5,17

Objectives

Methods

Conclusion