Cell proliferation and integration by micron/sub-micron surface roughness on Ti6Al4V

Abstract

Event Abstract Back to Event Cell proliferation and integration by micron/sub-micron surface roughness on Ti6Al4V Pavan Kumar Srivas1, Kausik Kapat1*, Bodhisatwa Das1*, Prabhash Dadhich1, Pallabi Pal1 and Santanu Dhara1 1 Indian Institute of Technology Kharagpur, School of Medical Science & technology, India Introduction: Surface properties of a metallic implant play an important role in the regulation of the interactions between biological environments (e.g. proteins, cells, tissues, etc.) and implants. Titanium and its alloys Ti6Al4V have been widely used as dental implants, artificial joints and bone fixation devices, due to its mechanical property, good corrosion resistant and biocompatibility[1],[2]. Moreover, Ti6Al4V as a material too satisfies the physico-chemical factors needed like corrosion resistance, hardness, density, high wear resistance and high strength[3] etc. Surface roughness promotes cell adhesion promoting better fixation and integration of implants. In this study, micro roughness has been generated by creating different shapes of micro pillars/grooves on Ti6Al4V to enhance cell attachment and tissue adhesion. Material and methods: Two type of micro-patterened Ti6Al4V samples like pillar and groves were developed from Ti6Al4V powders, particle size of 15-45 µm (Good Fellow, U.K.) via powder metallurgy route. samples were completely dried in oven prior to sintering. Sintering was performed at 1400°C for all the samples in presence of argon gas. The sintered samples were evaluated by scanning electron microscopy (SEM), streozoom microscope. Mechanical testing was done to measure the strength and toughness of the scaffold. MSCs, cell line, has been to test the cytocompatibility as well as cell proliferation property of the Ti6Al4 scaffold. MTT and live/dead assay was done to evaluate scaffold cytocompatibility. In vivo studies using rabbits were also performed to assess scaffold biocompatibility. Results and discussion: Hexagonal pillers and groves were succesfully developed on the surface of the Ti6Al4V scaffold. Preliminary results using SEM showed significant cell attachment and proliferation while MTT and live/dead assay indicated non-toxic nature of the matrix. Animal study results revealed excellent tissue adheshion on Ti6Al4V sample surface. Conclusion: In vitro study as well as in vivo assessment for micropatterned Ti6Al4V surface were confirmed significantly cell attachment, proliferation and tissue adhesion. Defence Research and Development Organisation, Ministry of Defence, India.