Cell Progeny in the Olfactory Bulb After Targeting Specific Progenitors with Different UbC-StarTrack Approaches.

Rebeca Sánchez-González,María Figueres-Oñate,Laura López-Mascaraque,Ana Cristina Ojalvo-Sanz

doi:10.3390/genes11030305

Rebeca Sánchez-González, María Figueres-Oñate + Show 2 more

Open Access

https://doi.org/10.3390/genes11030305

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Journal: Genes	Publication Date: Mar 13, 2020
Citations: 7	License type: CC BY 4.0

Affiliation: Instituto Cajal

Abstract

The large phenotypic variation in the olfactory bulb may be related to heterogeneity in the progenitor cells. Accordingly, the progeny of subventricular zone (SVZ) progenitor cells that are destined for the olfactory bulb is of particular interest, specifically as there are many facets of these progenitors and their molecular profiles remain unknown. Using modified StarTrack genetic tracing strategies, specific SVZ progenitor cells were targeted in E12 mice embryos, and the cell fate of these neural progenitors was determined in the adult olfactory bulb. This study defined the distribution and the phenotypic diversity of olfactory bulb interneurons from specific SVZ-progenitor cells, focusing on their spatial pallial origin, heterogeneity, and genetic profile.

Highlights

The mammalian olfactory system is composed of the olfactory epithelium (OE), olfactory bulb (OB), and olfactory cortex (OC)
Mitral cells in the mitral cell layer (MCL). Were identified through their morphology and the presence of reelin. These results indicated that glial and mitral cells originated from progenitor cells located in the most rostral part of the lateral ventricle (LV) at E12
A novel StarTrack approach was adopted to address the ontogeny of different cell types in the adult rodent OB, taking into account the identity of their progenitor cells and their location in the LV

Summary

Introduction

The mammalian olfactory system is composed of the olfactory epithelium (OE), olfactory bulb (OB), and olfactory cortex (OC). Using the Golgi method, Santiago Ramón y Cajal described the layers in the OB and its components more than a century ago (Figure 1A reproduces an original drawing of Cajal [3]; reviewed in [4]). His morphological studies on the OB provided the basis to define the neurons present in this structure and when UbC-StarTrack strategy is compared, the cells labeled in the adult OB following in utero electroporation (IUE) are similar to those drawn by Cajal (Figure 1A–F). The axonal projections of mitral cells form the lateral olfactory tract (LOT) and they establish direct contacts with the OC [6,7]

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