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Abstract
The successful incorporation of biomaterials implanted in the human body depends on many factors, amongst which are the surface properties of the solid implant, the surrounding liquid with its dissolved proteins and the surface characteristics of cells. Under physiological conditions, biomaterials are immediately covered with proteins before cells can adhere to the material, thereby changing the original characteristics of the substratum surface. Cell spreading was significantly better on high surface free energy substrata than on low energy substrata, both in the absence and in the presence of preadsorbed proteins, despite the fact that a small convergence of the surface free energies of the various substrata occurred upon adsorption. Adsorbed proteins thus seem to transfer substratum characteristics towards adhering and spreading cells. Fourier transform infrared (FTIR) spectra of proteins adsorbed on various substrata indeed demonstrated the appearance of the amide I and amide II bands, with minor shifts in the wave numbers and small changes in the shape of the absorption bands. These band shifts might indicate conformational changes of the adsorbed protein layers, which are probably responsible for the transfer of substratum properties to the interface with adhering and spreading cells.
Published Version
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