Abstract
Cell compartmentation of EcoRI endonuclease was analyzed using either parental or tolA excretory strains of Escherichia coli. Cells were subjected to various fractionation procedures such as osmotic shock or spheroplast formation. Our results showed that EcoRI activity was almost entirely recovered into cytoplasmic fractions and consequently was not released into the extracellular medium by a tolA mutant. These results did not support previous reports suggesting a periplasmic location for the EcoRI enzyme and did not allow to develop a simple method for EcoRI purification from culture supernatants of excretory mutants.
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