Abstract
This tutorial describes a method of controlled cell labeling with citrate-coated ultra small superparamagnetic iron oxide nanoparticles. This method may provide basically all kinds of cells with sufficient magnetization to allow cell detection by high-resolution magnetic resonance imaging (MRI) and to enable potential magnetic manipulation. In order to efficiently exploit labeled cells, quantify the magnetic load and deliver or follow-up magnetic cells, we herein describe the main requirements that should be applied during the labeling procedure. Moreover we present some recommendations for cell detection and quantification by MRI and detail magnetic guiding on some real-case studies in vitro and in vivo.
Highlights
Different strategies can be applied in order to endow cells with sufficient magnetization to be detectable by magnetic resonance imaging (MRI) and/or to be manipulated by an external magnetic field
Iron oxide nanoparticles as cellular MRI contrast agents In order to be distinguished from tissues, the cells have to be labeled with a contrast agent (Figure 8)
Summary and conclusion Iron oxide nanoparticles can be used for magnetic labeling of different types of cells
Summary
Different strategies can be applied in order to endow cells with sufficient magnetization to be detectable by MRI and/or to be manipulated by an external magnetic field. In order to efficiently and safely label different cell types, including stem cells, this tutorial presents a well-established method of controlled cell labeling with citrate-coated ultra small superparamagnetic iron oxide nanoparticles ( referred to as magnetic nanoparticles - MNP).
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