Cell-imaging studies of highly substituted oxazole derivatives as organelle targeting fluorophores (OTFPs)

Abstract

The field of biomedical science has progressed enormously in the past decade. With the advent of newer diagnostic tools for imaging and detection, identification of root cause of a disease is now remarkably accurate and specific. Small organic fluorophores in these connections are in great demand currently for cellular organelle sensing and detecting, due to their non-invasiveness, excellent accuracy and bio-sensitivity. Small molecule fluorescence probes offer most potent area for biological sensing with diagnostic imaging ability. These organelle targetable fluorescent probes are produced through synthetic manipulations to get the desired, decent fluorescence properties. When a suitable organelle specific functional group is installed within these highly fluorescent scaffolds, then these molecules turn out to be as lysotracker, mitotracker and cytoplasm-stainer in mammalian cells with high efficiencies (high Pearson co-efficient factors). The present work demonstrated an environmentally benign (green) one-pot, sp3 C–H functionalization of highly substituted oxazole derivatives with excellent photophysical properties. These molecules were further modified by installing organelle specific targetable groups (sensors/detectors) which selectively localize in specific intra-cellular organelles.