Abstract
A suspension culture of Eucalyptus tereticornis was initiated from callus and grown for 7 months under indirect light in a Murashige and Skoog (1962) basal medium containing 3% glucose and 1 mg/l 2,4-D. Glucose was used, instead of sucrose, as it reduced production of phenolic-like compounds. The inoculum size for maximum cell yield was determined. Cells (0.1, 0.2, 0.5, 1.0, 2.5 and 5.0 g fresh weight) were cultured in basal medium for 14 days. Maximum fresh weight (mean 11.8 g) was attained from samples inoculated with at least 1.0 g of cells. Largest dry weight (mean 608 mg) occurred following, inoculation with at least 0.5 g fresh weight of cells. Inoculation with 0.5 g of cells resulted in the most rapid fresh weight doubling time (3.4 days).After 17 months of culture, cells were grown in basal medium or m basal medium supplemented with 1 mg/l kinetin, under continuous, direct light. Growth, based on fresh and dry weight increases, was measured over the 2-week subculture period. Growth of cells was similar in both media. The cells' chlorophyll content remained low. Fresh weight doubling time averaged 3.8 days.
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