Abstract
The Thiobacillus thiooxidans S3 strain was grown on modified Silverman 9K medium containing thiosulfate as a sole energy source instead of sulfur. When the pH of medium was controlled at 5.0 during the cultivation, the growth rate greatly increased to 0.067h-1 and the cell yield obtained with 1% thiosulfate was 210mg/l. When cell growth declined, placing the culture in fresh medium effectively prolonged the period of linear growth and the cell concentration reached 891mg/l in 309h of cultivation. The cells obtained in the replacement culture efficiently oxidized the sulfite.
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