Abstract
This report describes an effective method for single-step isolation of cell line variants with reduced intercellular plasma membrane fusion ability. Variants, derived from a subline of L5178Y, were obtained by selection for growth in the presence of a toxic level of PEG 6000. These variants occurred after EMS treatment and spontaneously. Further testing revealed a pleiotropic phenotype, fusion impairment and polyethylene glycol resistance, which has been designated PgfR. Variant sublines, in serial subculture for more than 165 days on nonselective medium, retain a stable PgfR phenotype. PgfR variants have an approximate 7-fold reduction in intercellular fusion competence.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
