Cell-free transmission of mouse neuroblastoma.

Abstract

AFTER the description of the cell-free transmission of neuroblastoma C1300, a transplantable tumour in strain A/J mice, by Prasad et al.1, we made ten attempts to reproduce their results, first using their extraction procedure and then by using the viral concentration procedure of Moloney2, modified by Huebner et al.3. We inoculated twenty-eight prenatal, forty-seven newborn, and sixteen 4-week-old A/J mice but failed to obtain positive results, even after 100 days postinoculation. Because we used newborn mice that are usually more susceptible to oncogenic viruses than the older mice used by Prasad et al., and viral concentrates rather than filtrates, we expected to increase our chances of transmission.