Abstract
Abstract Messenger RNA extracted from polysomes of KB cells at late stages of productive infection with adenovirus type 2 was translated in a cell-free system derived from Krebs II ascites cells. Four of the polypeptides obtained in this reaction corresponded to the virion coat components hexon, IIIa, penton base, and fiber. The following criteria were used to establish identity between the in vitro and in vivo products: (a) comigration in sodium dodecyl sulfate acrylamide gel electrophoresis; (b) co-chromatography on sodium dodecyl sulfate hydroxyapatite; (c) specific immunoprecipitation; and (d) matching tryptic peptide fingerprints.
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