Abstract
AbstractIn current cell‐free biotechnology, mimicking physicochemical aspects of cellular life while providing efficient protein synthesis, separation, and purification is a crucial step towards truly applicable artificial, yet cell‐like, biosynthesis platforms. To achieve simultaneous synthesis and immobilization of proteins in a tailor‐made environment, we link DNA encoding for histidine‐tagged proteins to microfluidically prepared microgels via strain‐promoted azide‐alkyne cycloaddition and introduce nitrilotriacetic acid moieties that exhibit affinity towards histidine‐tagged proteins upon nickel activation. After swelling the microgels in a prokaryotic protein synthesis machinery, proteins are produced and remain at the site of synthesis despite the membrane‐free, open nature of the gels. The protein‐loaded microgels are then separated from the complex bioreaction mixture and proteins are released by a chemical trigger. Our bifunctional microgels not only contribute to the ongoing design of artificial cells but also allow for directly linking proteins to specific DNA, which can be explored for genotype‐phenotype correlation in DNA mutagenesis.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
