Cell Differentiation and Replication during Postnatal Development of the Murine First Molar.

Rudi Balzano,Carla Mucignat-Caretta,Edoardo Stellini

doi:10.3390/biology10080776

Rudi Balzano, Carla Mucignat-Caretta + Show 1 more

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https://doi.org/10.3390/biology10080776

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Abstract

Simple SummaryTeeth are necessary to prepare food for swallowing. The process of teeth development before and after birth may be studied in normal mice and also by reproducing diseases or genetic conditions. However, mice teeth are different from human teeth, since mice have only permanent teeth. Moreover, their incisors continue to grow for the whole lifespan. Hence, it is important to know how the mouse teeth develop. We studied the development of the first molar in mice from birth to weaning and showed that dividing cells are located in a different part of the developing tooth according to age.Various signaling molecular pathways are involved in odontogenesis to promote cellular replication and differentiation. Tooth formation is controlled mainly by epithelial–mesenchymal interactions. The aim of this work was to investigate how cellular replication and differentiation ensue during the formation of the murine first molar in postnatal ages until eruption, focusing on morphogenesis, odontoblast differentiation and cellular replication. Wild-type CD1 mice were examined from birth to weaning. Morphogenesis and interaction between developing epithelial and mesenchymal tissues were evaluated in hematoxylin–eosin and Gomori trichome stained sections. Immunohistochemistry for nestin, which mediates the differentiation of odontoblasts, especially their polarization and elongation, showed that this intermediate filament was apparent already at postnatal day P1 in the apical region of odontoblasts and progressed apically from cusp tips, while it was not present in epithelial tissues. The expression of nuclear antigen Ki-67 highlighted dividing cells in both epithelial and mesenchymal tissues at P1, while one week later they were restricted to the cementoenamel junction, guiding root elongation. The link between odontoblast maturation and cellular replication in the different tooth tissues is essential to understand the development of tooth shape and dimension, to outline mechanisms of tooth morphogenesis and possibly eruption.

Highlights

Odontogenesis is an example of complex interactions among different tissue components
Dentin deposition at P1 starts from the cusp tip (Figure 1A), with the dentin layer becoming thinner in the apical direction
The mouse as an experimental model is of utmost importance as a research tool for elucidating the mechanism of odontoblast differentiation and cellular replication in postnatal odontogenesis

Summary

Introduction

Odontogenesis is an example of complex interactions among different tissue components. Understanding its time course may cast light on general processes relevant to development of different organs. It recently became a major focus for developmental research. Teeth development starts from an epithelial thickening, the dental lamina, which in the mouse originates during embryonal day 12 (E12) [4], giving rise in both the maxilla and mandible to two incisors that grow throughout the mouse’s life and show a remarkable cell heterogeneity [5]. The teeth development process goes through a series of stages called bud, cap and bell [6], involving epithelial remodeling through differential growth rate and apoptosis and variations in cell shape driven by cell–cell and cell–matrix interactions

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