Cell death unlikely contributes to taxol production in fungal elicitor-induced cell suspension cultures of Taxus chinensis.

Abstract

Cell suspension cultures of Taxus chinensis, with 20, 40 and 100 mg fungal elicitor l(-1) from Aspergillus niger, underwent rapid cell death after 24 h, which was about 2, 3.7 and 5-fold of that of the control. At the same time, Taxol production was increased, respectively, to about 5, 8 and 3-fold of that of the control. Inhibition of phenolics biosynthesis resulted in a 150% increase in cell death but a 54% decrease in Taxol production compared with 40 mg elicitor l(-1) alone. O2-free N2 inhibited cell death but had little effect on Taxol production as induced by 40 mg fungal elicitor l(-1).