Abstract
Ultrahigh-resolution optical coherence tomography (UR-OCT) has been used for the first time to our knowledge to study single-cell basal cell carcinoma (BCC) in vitro. This noninvasive, in situ, label-free technique with deep imaging depth enables three-dimensional analysis of scattering properties of single cells with cellular spatial resolution. From three-dimensional UR-OCT imaging, live and dead BCC cells can be easily identified based on morphological observation. We developed a novel method to automatically extract characteristic parameters of a single cell from data volume, and quantitative comparison and parametric analysis were performed. The results demonstrate the capability of UR-OCT to detect cell death at the cellular level.
Highlights
The aim of cancer therapies is mainly to stop cell proliferation or induce cell death
We report the detection of cell death at the single-cell level using ultrahigh-resolution optical coherence tomography (UR-OCT)
The viability of live cells was confirmed by positive calcein staining by confocal microscopy (Fig. 3b) before they were imaged with UR-OCT
Summary
The aim of cancer therapies is mainly to stop cell proliferation or induce cell death. Some cancer cells are killed, but others remain resistant This cell-to-cell variability might be attributed to genetic, epigenetic or proteomic factors, the presence of cancer stem cells and different stages of the cell cycle [5,6,7,8,9,10]. Measuring the response at the single-cell level provides further pharmacokinetic and pharmacodynamic information, which aids drug development and regimen design [11,12]. Fluorescence microscopy can be used to detect cell death at the single-cell level after cancer cells have been labeled by genetically engineered reporters or exogenous fluorescent dyes [13,14,15,16]. We report the detection of cell death at the single-cell level using ultrahigh-resolution optical coherence tomography (UR-OCT)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
