Abstract
The role of prostaglandin 12 (PGI 2) in the control of DNA synthesis during the cell cycle was investigated in cultured rabbit aortic smooth muscle cells (SMC). SMC at confluency in the G 0 state reached the S phase about 16 h after stimulation with serum, as judged by measurement of [ 3H]thymidine incorporation into DNA (DNA synthesis). Cyclooxygenase inhibitors such as indomethacin and aspirin enhanced DNA synthesis, suggesting that endogenously synthesized prostaglandins inhibit DNA synthesis. Added PGE 1 or PGE 2 had little effect on DNA synthesis. PGI 2 inhibited DNA synthesis only when added from 10 to 16 h after stimulation of SMC in the G 0 state with serum. Addition of CS-570, a stable PGI 2 analogue, inhibited DNA synthesis at any time after serum stimulation. The endogenous syntheses of (PGI 2) and DNA were negatively correlated. These results suggest that PGI 2 inhibits DNA synthesis by acting on the progression stage of the G 1 state.
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