Abstract
HL-60 leukemia cells were fractionated into G 1 and S G 2 populations using a rapid centrifugal elutriation technique, and studied for differences between the cell-cycle phases. The G 1 fraction was found to contain smaller cells with a sedimentation velocity of 7 mm/h. The S G 2 fraction consisted of larger cells with a sedimentation velocity of 125 mm/h. The latter fraction was found to have a peak level of the enzyme (2′-5′)A n -binding protein, as compared to the G 1 fraction, indicating a possible role for (2′-5′)A n -binding protein and its products in the growth regulation of these leukemic cells. In addition, cytofluorometric analysis of fractionated HL-60 cells indicates that elutriation is an effective fractionation method, rapidly yielding large numbers of cells for study, without the use of chemical treatments.
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