Cell cycle dependent changes in morphology: Studies with a cold-sensitive mutant of Chinese hamster ovary cells

Abstract

cs4-D3, a cold-sensitive mutant of CHO cells is defective at the G1 interval of the cell cycle and exhibits a reversible change in morphology from a fibroblast appearance to an “epithelial-like” form. Here, we have shown by time-lapse microcinematography that change in morphology, in either direction, is a post-mitotic event.