Abstract
Adriamycin fluorescence was measured by flow cytometry after exposing synchronized Chinese hamster (line CHO) cells to a fixed dose of adriamycin (ADR). Three synchrony methods were used: mitotic selection, isoleucine-deficient culture and pretreatment with low doses of ADR. Approximately a 1.6- to 2-fold increase in ADR fluorescence intensity was observed for cells in G 2 + M compared to cells in G 1. An increase in ADR fluorescence was also noted for cell populations exhibiting a G 2 block after pretreatment with low-dose ADR. No significant difference in fluorescence intensity was observed for G 1 cell populations with two different volume distributions. Results suggest that the magnitude of ADR fluorescence is affected by the cell cycle distribution and that cellular susceptibility to drug-induced cytokinetic changes during exposure must also be taken into consideration when measuring ADR uptake.
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