Abstract
Hybrid liposomes (HL), composed of L-α-dimyristoylphosphatidylcholine and polyoxyethylene(23) dodecyl ethers, were simply prepared by the sonication method. In this study, we investigated the effects of HL on cell cycle and apoptosis in human non-small cell lung cancer cells. Induction of cell cycle arrest at the G0/G1 phase and apoptosis by HL were observed in human non-small cell lung cancer cells (A549, H460 and H23). HL treatment also resulted in the induction of cyclin-dependent kinases inhibitor p21WAF1/CIP1 and p27KIP1 and a decrease in the protein expressions of cyclins D1 and E. It is noteworthy that the treatment of A549 cells with HL inhibited phosphorylation of Akt in a time- and dose-dependent manner. Furthermore, HL treatment inhibited the filopodia formation in A549 cells. These results suggest that HL-induced cell cycle arrest at the G0/G1 phase could be associated by the up-regulation of cdk inhibitor p21 and p27 through blocking Akt signaling
Highlights
Non-small cell lung carcinoma (NSCLC), including adenocarcinoma, squamous-cell lung carcinoma, and large-cell lung carcinoma, is the most common form of lung cancer and accounts for the most deaths of any cancer worldwide
We have reported that hybrid liposomes (HL) composed of 90 mol% DMPC and 10 mol% C12(EO)23 induced apoptosis in various cancer cells including NSCLC (A549, H460, H23 and H520) cells [9,13,14,15]
We investigated the effects of HL composed of 90 mol% DMPC and 10 mol% C12(EO)23 on the cell cycle and apoptosis of NSCLC (A549, H460 and H23) cells in vitro, and found the induction of cell cycle arrest at G0/G1 phase through blocking Akt signaling along with apoptosis
Summary
Non-small cell lung carcinoma (NSCLC), including adenocarcinoma, squamous-cell lung carcinoma, and large-cell lung carcinoma, is the most common form of lung cancer and accounts for the most deaths of any cancer worldwide. We have reported that HL composed of 90 mol% DMPC and 10 mol% C12(EO) induced apoptosis in various cancer cells including NSCLC (A549, H460, H23 and H520) cells [9,13,14,15]. We have reported that HL inhibits the growth of colorectal cancer (HCT116) cells through the induction of cell cycle arrest at G0/G1 phase along with apoptosis [16]. We investigated the effects of HL composed of 90 mol% DMPC and 10 mol% C12(EO) on the cell cycle and apoptosis of NSCLC (A549, H460 and H23) cells in vitro, and found the induction of cell cycle arrest at G0/G1 phase through blocking Akt signaling along with apoptosis
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