Cell cycle arrest by cowanin on T47D breast cancer cell line

Abstract

Event Abstract Back to Event Cell cycle arrest by cowanin on T47D breast cancer cell line Fatma S. Wahyuni1*, Dessy Arisanty1 and Lusiana N. Yusra1 1 Andalas University, Indonesia Background Breast cancer is one of the most common cause of cancer deaths each year. The incidence of cancer is very closely related to cell cycle. Cancer is a disease in which there are irregularities in the regulation of cell cycle. Treatment of cancer depends mainly on chemotherapy. Nevertheless, anticancer agents lack selectivity and are often compromised by onset of resistance. Methods The search for new drugs from natural sources is required. This study investigated the effect of cowanin from the stem bark of asam kandis (Garcinia cowa Roxb.) against T47D breast cancer cell cycle by flow cytometry method. Previous study showed that cowanin induced cytotoxic effect at IC50 11.68 µg/mL against T47D breast cancer cells. This study involved three groups, namely the control group, treatment group 1 (cowanin at 1x IC50), and treatment group 2 (2x IC50). Results Flow cytometry analysis showed that cowanin inhibited T47D breast cancer at G0-G1 phase. Concentration of 1x IC50 yielded an average percentage of inhibition 54.12% whereas the concentration of 2x IC50 produced an average percentage of inhibition 67.72%. Statistical analysis with one way ANOVA showed that there is significant difference between each treatment group in the percentage of inhibition on G0-G1 phase. Conclusion The results indicated that cowanin induced cell cycle arrest in T47D cells, suggesting its potential as a new chemotherapeutic agent Acknowledgements The author would like to say special thanks to The Dean of Faculty of Faculty of Pharmacy, Andalas University, Padang, Indonesia for his support this research under Lecturer Research Grant, Fiscal Year 2017. Keywords: Cowanin, Garcinia cowa, Breast cacner, Cell cyle, flow cytoametry, T47D Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Oral Presentation Topic: Cancer Citation: Wahyuni FS, Arisanty D and Yusra LN (2019). Cell cycle arrest by cowanin on T47D breast cancer cell line. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00124 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 30 Nov 2018; Published Online: 17 Jan 2019. * Correspondence: Prof. Fatma S Wahyuni, Andalas University, Padang, Indonesia, fatmasriwahyuni@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Fatma S Wahyuni Dessy Arisanty Lusiana N Yusra Google Fatma S Wahyuni Dessy Arisanty Lusiana N Yusra Google Scholar Fatma S Wahyuni Dessy Arisanty Lusiana N Yusra PubMed Fatma S Wahyuni Dessy Arisanty Lusiana N Yusra Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.