Cell cycle analysis in bone marrow and kidney tissues of dietary restricted rats

Abstract

The effect of dietary restriction (DR) on the proportion of cells in various phases of the cell cycle as determined by flow cytometry was investigated in the bone marrow and kidney of young and old Fischer 344 rats. Control rats were fed a standard occurrence of numerous age-associated diseases, including cancer, renal diseases and by the control rats starting at 16 weeks of age until killed at 5 or 20 months old. The relative proportion of cells in the various phases of the cell cycle was independent of tissue type, treatment condition and age, consistently showing an order of G 1- > S- > G 2M-phase. In old rats DR did not affect cell cycling in bone marrow of either sex, however, it did cause an increase in the percentage of G 1-phase cells in the kidney of male rats. Additionally, DR caused a mathematically significant change in the percentage of cells in all phases of the cell cycle in the bone marrow of young male rats but had no effect in young females. The percentage of S-phase cells in both tissues of both sexes decreased in old rats when compared to young rats regardless of treatment conditions, indicating a parallel decline in cell proliferating activity with aging. To summarize, DR produces a greater cell cycle effect in the young male than the old male rats. Proliferative capacity is enhanced when the young male rats are dietary restricted. This may aid in DNA repair mechanisms and/or immune system response.