Abstract
This paper describes the separation of rumen mucosa cells by pretrypsinization with 0.1% trypsin elusion solution composed of 2% sodium citrate, 2% EDTA-Na, 0.001% Tween 80 and 0.01% polybrene in 0.9% sodium chloride, pH 7.2. In the use of the cells separated following trypsin-EDTA treatment, three culture cases are described. Key words: Cell culture, rumen mucosa, bovine
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