Cell Chromatography-Based Screening of the Active Components in Buyang Huanwu Decoction Promoting Axonal Regeneration.

Xiangli Yan,Xiao Shen,Lisheng Wang,Haozhen Zheng,Aiming Yu,Shengxin Wang

doi:10.1155/2019/6970198

Abstract

Buyang Huanwu decoction (BHD), a popular formulation prescribed in traditional Chinese medicine (TCM) for the treatment of ischemic stroke, has been reported to have a potential role in promoting axonal regeneration. The purpose of the study was to screen and identify bioactive compounds from BHD using live PC12 cells coupled with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Using this approach, we successfully identified six bioactive components from BHD. These components have protective effects on oxygen-glucose deprivation/reperfusion (OGD/R) injury to PC12 cells. Furthermore, calycosin-7-d-glucoside (CG) and formononetin-7-O-β-d-glucoside (FG) could upregulate the protein expression of growth-associated protein 43 (GAP-43) and brain-derived neurotrophic factor (BDNF). This study suggests that living cells combined with HPLC-MS/MS can be used for the screening of active ingredients in TCMs.

Highlights

Ischemic stroke is a brain disease marked by insufficient blood supply to brain tissues, with high morbidity and mortality.e limited degree of spontaneous recovery after stroke translates into profound economic and emotional burdens
We investigated the protective effects of calycosin-7-O-β-D-glucoside and formononetin7-O-β-D-glucoside on PC12 cells with injury by detecting the expression of growth-associated protein 43 (GAP-43) and brain-derived neurotrophic factor (BDNF) proteins
We investigated the protective effects of calycosin-7-O-β-Dglucoside and formononetin-7-O-β-D-glucoside on PC12 cells with injury by detecting the expression of GAP-43 and BDNF proteins. e results showed that calycosin-7-Oβ-D-glucoside and formononetin-7-O-β-D-glucoside could promote axonal regeneration by upregulating the expression of GAP-43 and BDNF proteins

Summary

Introduction

Ischemic stroke is a brain disease marked by insufficient blood supply to brain tissues, with high morbidity and mortality.e limited degree of spontaneous recovery after stroke translates into profound economic and emotional burdens. Axonal injury blocks normal cell-to-cell interactions or neural circuits in the ischemic brain, which usually occurs before neuronal death due to energy depletion or cerebral edema [1]. The brain has some ability to compensate for the functions lost after a stroke owing to the remarkable plasticity of this organ [4]. A large body of evidence suggests that axonal regeneration plays a key role in the recovery of brain function because it can establish new neural connections to alleviate brain function loss caused by stroke [5,6,7]. BHD administration following spinal cord injury has been reported to protect injured neurons, promote regeneration, and enhance functional recovery [8]. BHD has been demonstrated to boost axonal regeneration effects, the effective components of BHD exerting the neuroprotective effects still need to be investigated

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