Abstract
RATIONALE: Basophil activation and desensitization have been largely characterized by traditional bulk assays (e.g., mediator release, phopho-specific immunoassays). However, these methods are not able to resolve events on the individual cell level, nor are they useful for monitoring basophil activation in patients. Our objective was to develop flow cytometic methods for studying basophil activation and the mechanisms of non-specific desensitization that can be applied to human studies.
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